Open AccessIdentification of carbapenemases enterobacteriaceae producing gene blaVIM in clinical isolates
Author(s) -
Ella Amalia,
Tia Sabrina,
Yuwono,
Venny Patricia,
Radhiyatul Husna,
Ayeshah Augusta Rosdah,
Safyudin
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1246/1/012004
Subject(s) - klebsiella pneumoniae , gene , biology , polymerase chain reaction , enterobacteriaceae , microbiology and biotechnology , genotype , genetics , escherichia coli
Carbapenemase enzymes play the most important role in the existence of CRE (C arbapenem-resistant Enterobacteriaceae ). VIM is one of the carbapenemase enzymes encoded by the bla VIM gene. The genes which are partially located in genetic mobile elements may facilitate the spread of the resistance to other bacteria. The aim of this study was to identify the bla VIM gene in CRE isolated from infected patients in dr. Mohammad Hoesin Palembang. This descriptive observational laboratory study using 709 samples of Enterobacteriacea e isolated from various specimens of infected patients at dr. Mohammad Hoesin Palembang during the September-November period which would be identified as CRE using Vitek 2 Compact. 21 isolates which have been identified by CRE was taken by purposive sampling method to detect bla VIM gene with conventional polymerase chain reaction (PCR). The PCR product was electrophoresed and visualized to see a single 390bp DNA band. Out of 709 isolates of Enterobacteriacea e, 88 (12.4%) isolates were identified as CRE. Only 21 isolates of CRE performed PCR. Three (14.3%) isolates carrying the blaVIM gene which found in Enterobacter sp . (N=2; 66.7%) and Klebsiella pneumoniae (n=1; 33.3%). In this study 14.3%, Enterobacteriacea e with CRE phenotype has bla VIM gene genotype.