Open AccessA New Spectrophotometric Method to Determine Vitamin B6 in Pharmaceutical Formation Samples Using a Micelle Form
Author(s) -
Ahmed Fadhil Khudhair,
Shaymaa Ibrahim Saeed,
Ashraff Aziz Marhoon,
Hasan F. Alesary
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1234/1/012087
Subject(s) - pyridoxine hydrochloride , calibration curve , detection limit , reagent , chemistry , vitamin b6 , chromatography , salting out , pulmonary surfactant , pyridoxine , dosage form , micelle , analytical chemistry (journal) , vitamin , aqueous solution , organic chemistry , biochemistry
An economic and sensitive method was developed to measure pyridoxine hydrochloride (vitamin B6) in pharmaceutical formation as an ion pair, as depending on the charge transfer reaction with SDS as a surfactant and a suitable analytical reagent (chlorazol black). The parameters that gave optimum reaction conditions, such as the concentrations of chlorozal black, SDS, pH, equilibration temperature, time and effect of salting were studied to obtain a linear calibration curve where the linearity range was found to lie between 1.22 × 10 -3 to 34 × 10 -2 mM, and the detection limit (LOD) 2.56 × 10 -4 mM. The method was applied successfully to determine vitamin B6 concentrations in various pharmaceutical samples.