Open AccessScreening of potential isolate candidates probiotic against Aeromonas hydrophila from Boyolali, Indonesia
Author(s) -
Sarjito Sarjito,
Alfabetian Harjuno Condro Haditomo,
Restiana Wisnu Ariyati,
Aninditia Sabdaningsih,
. Desrina,
Slamet Budi Prayitno
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1217/1/012147
Subject(s) - aeromonas hydrophila , bacillus (shape) , biology , microbiology and biotechnology , catfish , aeromonas , bacteria , probiotic , outbreak , fish <actinopterygii> , fishery , virology , genetics
Mass mortality in catfish culture frequently occurs in Boyolali as a central production due to the outbreak of bacterial diseases. The main causative agent of bacterial disease is Aeromonas hydrophila. This research aimed to find out the bacteria isolates were potential against A. hydrophila . The exploratory method was commenced. Thirty-four isolates were gained from water (SBA01–SBA14) and mud (SBL01 – SBAL20) that were collected from the fish pond of Boyolali Regency, Indonesia with TSA medium. Screening the potential bacteria candidates against A.hydrophila using the sensitivity test that was conducted with in vitro method. Based on the screening results showed that three isolates (SBA14, SBL11, and SBL20) were potential candidates against A. hydrophila. On the basis of sequence 16S rDNA analysis, the result showed that SBA 14, SBL 11 and SBL 20 were closely related to Bacillus flexus, Bacillus subtillis , and Bacillus velezensis respectively.