Open AccessIdentification and determination of phenolic acids content in mango “golek” leaves ethanol extract
Author(s) -
A. M. Khasan,
Enny Fachriyah,
Dewi Kusrini
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1217/1/012050
Subject(s) - gallic acid , chemistry , maceration (sewage) , chromatography , high performance liquid chromatography , mangifera , ethanol , acid hydrolysis , hydrolysis , sodium hydroxide , organic chemistry , botany , antioxidant , materials science , composite material , biology
Ethanol extract from mango “golek” leaves (Mangifera indica L) was obtained by maceration in ethanol 96%. Phenolic acid was isolated from the extract by hydrolysis with and without a hydrolyzing agent (TH). Two different hydrolyzing agents were used separately, i.e., sulfuric acid (HA) and sodium hydroxide (HB). Identification of phenolic acid was made with TLC and High-performance liquid chromatography (HPLC). Determination of phenolic acid content was carried out using TLC Scanner. Standard phenolic acid was used as a control. Phenolic acid compounds were obtained in the HA, HB and TH isolate namely gallic acid. The total gallic acid content was 35.88% consisting of HA, HB and TH fraction of 3.46%; 12.42%; 19.98%.