Open AccessAntimicrobial Activity Test of Mangosteen Leaves Ethanol Extract (Garcinia mangostana Linn) Against Pseudomonas aeruginosa Bacteria
Author(s) -
Rochmanah Suhartati,
Fitri Apriyani,
Khusnul Khusnul,
D. P. Virgianti,
Mochamad Fathurohman
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1179/1/012167
Subject(s) - garcinia mangostana , pseudomonas aeruginosa , chemistry , ethanol , bacteria , antimicrobial , antibacterial activity , minimum inhibitory concentration , food science , pseudomonas , traditional medicine , microbiology and biotechnology , biology , biochemistry , medicine , organic chemistry , genetics
Pseudomonas aeruginosa causes various infections such as skin infections. Mangosteen leaves contains active compounds that are useful in inhibiting bacterial growth. The purpose of this research is to examine the antibacterial activity of mangosteen leaves ethanol extract ( Garcinia mangostana Linn ) on the growth of Ps . aeruginosa bacteria. The experiment was conducted by an experimental method of Ps . aeruginosa bacteria using the Kirby-Bauer method. The concentration of ethanol extract from mangosteen leaves studied is 10% to 100% concentration with a bacterial density of 0.5 Mc Farland. The results showed that the ethanol extract of mangosteen leaves could inhibit bacteria from a concentration of 10% with an average inhibitory diameter is 13.20 mm, 20% is 14.00 mm, 30% is 14.65 mm, 40% is 15.85 mm, 50% is 16.05 mm, 60% is 16.90 mm, 70% is 17.55 mm, 80% is 18.75 mm, 90% is 19.25 mm and 100% equal to 24.80 mm. Based on the results of research and analysis date that has been carried out, it can be concluded that the ethanol extract of mangosteen leaves ( Garcinia mangostana Linn ) has antibacterial activity against Ps . aeruginosa , with a minimum inhibitory concentration of 10% with diameter inhibitor zone is 13.20 mm.