Open AccessIn vitrophotoacoustic sensing of calcium dynamics with arsenazo III
Author(s) -
Nicholas Dana,
Robert Fowler,
Alicia C.B. Allen,
Janet Zoldan,
Laura J. Suggs,
Stanislav Emelianov
Publication year - 2016
Publication title -
laser physics letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 72
eISSN - 1612-202X
pISSN - 1612-2011
DOI - 10.1088/1612-2011/13/7/075603
Subject(s) - depolarization , photoacoustic imaging in biomedicine , calcium , materials science , contrast (vision) , imaging phantom , biophysics , penetration depth , signal (programming language) , biomedical engineering , optics , medicine , physics , biology , metallurgy , computer science , programming language
Imaging of cellular electric potential via calcium-ion sensitive contrast agents is a useful tool, but current it lacks sufficient depth penetration. We explore contrast-enhanced photoacoustic (PA) imaging, using Arsenazo III dye, to visualize cardiac myocyte depolarization in vitro . Phantom results show strong linearity of PA signal with dye concentration ( R 2 > 0.95), and agree spectrally with extinction measurements with varying calcium concentration. Cell studies indicate a significant (> 100-fold) increase in PA signal for dye-treated cells, as well as a 10-fold increase in peak-to-peak variation during a 30-second window. This suggests contrast-enhanced PA imaging may have sufficient sensitivity and specificity for depth-resolved visualization of tissue depolarization in real-time.