Open AccessQuantifying molecular- to cellular-level forces in living cells
Author(s) -
Jason Chao-Hsien Pan,
Tommy Kmieciak,
Yen-Ting Liu,
Matthew Wildenradt,
Yun Sheng Chen,
Yang Zhao
Publication year - 2021
Publication title -
journal of physics. d, applied physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.857
H-Index - 198
eISSN - 1361-6463
pISSN - 0022-3727
DOI - 10.1088/1361-6463/ac2170
Subject(s) - mechanosensitive channels , focus (optics) , living cell , nanotechnology , biology , microbiology and biotechnology , physics , materials science , genetics , ion channel , optics , receptor
Mechanical cues have been suggested to play an important role in cell functions and cell fate determination, however, such physical quantities are challenging to directly measure in living cells with single molecule sensitivity and resolution. In this review, we focus on two main technologies that are promising in probing forces at the single molecule level. We review their theoretical fundamentals, recent technical advancements, and future directions, tailored specifically for interrogating mechanosensitive molecules in live cells.