
A method for imaging single molecules at the plasma membrane of live cells within tissue slices
Author(s) -
Gregory I. Mashanov,
Tatiana A. Nenasheva,
Tatiana Mashanova,
Catherine Maclachlan,
N. J. M. Birdsall,
Justin E. Molloy
Publication year - 2020
Publication title -
the journal of general physiology/the journal of general physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.064
H-Index - 127
eISSN - 1540-7748
pISSN - 0022-1295
DOI - 10.1085/jgp.202012657
Subject(s) - membrane , biophysics , cytosol , microscopy , live cell imaging , cell membrane , microbiology and biotechnology , macromolecule , in situ , chemistry , cell , biology , biochemistry , physics , optics , organic chemistry , enzyme
Mashanov et al. present a method to image and track individual fluorescently labeled molecules in live cardiac tissue slices using total internal reflection fluorescence (TIRF) microscopy. They show that the movement and interaction of G protein–coupled receptors can be observed in real time.