Open AccessSingle-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis
Author(s) -
Satoshi Nakamizo,
Charles–Antoine Dutertre,
Ahad Khalilnezhad,
Xiao Meng Zhang,
Shawn Lim,
Josephine Lum,
Geraldine Koh,
Charlene S.F. Foong,
Pearly Yong,
Kahbing Jasmine Tan,
Reiko Sato,
Kaori Tomari,
Laurent YvanCharvet,
Helen He,
Emma GuttmanYassky,
Benoît Malleret,
Rintaro Shibuya,
Masashi Iwata,
Baptiste Janela,
Tsuyoshi Goto,
Tan Siyun Lucinda,
Mark Boon Yang Tang,
Colin Theng,
Valérie Julia,
Fériel HaciniRachinel,
Kenji Kabashima,
Florent Ginhoux
Publication year - 2021
Publication title -
the journal of experimental medicine/the journal of experimental medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.483
H-Index - 448
eISSN - 1540-9538
pISSN - 0022-1007
DOI - 10.1084/jem.20202345
Subject(s) - dendritic cell , psoriasis , cd14 , population , immunology , flow cytometry , inflammation , cell , t cell , biology , cell type , medicine , immune system , genetics , environmental health
Inflammatory skin diseases including atopic dermatitis (AD) and psoriasis (PSO) are underpinned by dendritic cell (DC)–mediated T cell responses. Currently, the heterogeneous human cutaneous DC population is incompletely characterized, and its contribution to these diseases remains unclear. Here, we performed index-sorted single-cell flow cytometry and RNA sequencing of lesional and nonlesional AD and PSO skin to identify macrophages and all DC subsets, including the newly described mature LAMP3+BIRC3+ DCs enriched in immunoregulatory molecules (mregDC) and CD14+ DC3. By integrating our indexed data with published skin datasets, we generated a myeloid cell universe of DC and macrophage subsets in healthy and diseased skin. Importantly, we found that CD14+ DC3s increased in PSO lesional skin and co-produced IL1B and IL23A, which are pathological in PSO. Our study comprehensively describes the molecular characteristics of macrophages and DC subsets in AD and PSO at single-cell resolution, and identifies CD14+ DC3s as potential promoters of inflammation in PSO.