Open AccessSingle-cell analyses identify circulating anti-tumor CD8 T cells and markers for their enrichment
Author(s) -
Kristen E. Pauken,
Osmaan Shahid,
Kaitlyn A. Lagattuta,
Kelly Mahuron,
Jacob M. Luber,
Margaret M. Lowe,
Lüping Huang,
Conor P. Delaney,
Jaclyn M. Long,
Megan E. Fung,
Kathleen Newcomer,
Katy K. Tsai,
Melissa Chow,
Samantha Guinn,
Juhi R. Kuchroo,
Kelly P. Burke,
Jason M. Schenkel,
Michael D. Rosenblum,
Adil Daud,
Arlene H. Sharpe,
Meromit Singer
Publication year - 2021
Publication title -
the journal of experimental medicine/the journal of experimental medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.483
H-Index - 448
eISSN - 1540-9538
pISSN - 0022-1007
DOI - 10.1084/jem.20200920
Subject(s) - cd8 , nkg2d , biology , t cell receptor , cytotoxic t cell , transcriptome , t cell , microbiology and biotechnology , single cell analysis , circulating tumor cell , cancer research , cell , immunology , antigen , immune system , gene , cancer , gene expression , genetics , in vitro , metastasis
Using single-cell RNA sequencing and TCR sequencing, Pauken et al. detect CD8 + T cell clones shared between blood and tumor in mice or melanoma patients, characterize matching clones in blood and tumor, and identify potential biomarkers for their isolation in blood.