Open AccessBorealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules
Author(s) -
Lin-Ing Wang,
Tyler Defosse,
Janet K. Jang,
Rachel A. Battaglia,
Victoria F Wagner,
Kim S. McKim
Publication year - 2021
Publication title -
the journal of cell biology/the journal of cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.414
H-Index - 380
eISSN - 1540-8140
pISSN - 0021-9525
DOI - 10.1083/jcb.202006018
Subject(s) - aurora b kinase , kinetochore , microbiology and biotechnology , biology , spindle checkpoint , spindle apparatus , spindle pole body , advanced spaceborne thermal emission and reflection radiometer , astral microtubules , microtubule , chromosome segregation , centromere , cell division , genetics , chromosome , physics , cell , satellite , astronomy , gene
The chromosomes in the oocytes of many animals appear to promote bipolar spindle assembly. In Drosophila oocytes, spindle assembly requires the chromosome passenger complex (CPC), which consists of INCENP, Borealin, Survivin, and Aurora B. To determine what recruits the CPC to the chromosomes and its role in spindle assembly, we developed a strategy to manipulate the function and localization of INCENP, which is critical for recruiting the Aurora B kinase. We found that an interaction between Borealin and the chromatin is crucial for the recruitment of the CPC to the chromosomes and is sufficient to build kinetochores and recruit spindle microtubules. HP1 colocalizes with the CPC on the chromosomes and together they move to the spindle microtubules. We propose that the Borealin interaction with HP1 promotes the movement of the CPC from the chromosomes to the microtubules. In addition, within the central spindle, rather than at the centromeres, the CPC and HP1 are required for homologous chromosome bi-orientation.