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Aspirin Ingestion Impairs Oral Mucosal Ulcer Healing by Inducing Membrane‐Bound Tumor Necrosis Factor‐a Release
Author(s) -
Slomiany Bronislaw L.,
Slomiany Amalia
Publication year - 2000
Publication title -
iubmb life
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/713803747
Subject(s) - citation , aspirin , oral medicine , impact factor , new england , medicine , library science , dentistry , law , political science , computer science , politics
Among the early manifestations of impairment by nonsteroidal anti-inflammatory drugs in mucosal tissue repair is the enhancement in tumor necrosis factor-alpha (TNF-alpha). We investigated the effect of aspirin ingestion on the processing of TNF-alpha in rat soft oral tissue during buccal ulcer healing. While in the control group, the ulcer healed by the 10th day; only a 54.8% reduction in the ulcer area was attained in the presence of aspirin administration. Moreover, by the 10th day, the delay in ulcer healing by aspirin was manifested in a 5.6-fold higher rate of apoptosis and a 5.2-fold higher level of soluble TNF-alpha, yet the expression of membrane-bound TNF-alpha showed a 38% decline. Treatment with metalloprotease inhibitor, Zincov, produced dose-dependent reduction (56.9%) in aspirin-induced increase in the mucosal expression of soluble TNF-alpha, evoked a 62% decrease in the rate of epithelial cell apoptosis, and led to a marked reversal (56.9%) in aspirin-induced delay in ulcer healing. Our findings indicate that the impairment in buccal ulcer healing by aspirin is a result of upregulation in the processing of soluble TNF-alpha from its membrane-bound precursor that leads to the amplification of apoptotic events and potentiation of the mucosal inflammatory responses that interfere with healing process.