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Cloning and Characterization of a Novel cDNA Sequence Encoding the Precursor of a Novel Venom Peptide (BmKbpp) Related to a Bradykinin‐Potentiating Peptide from Chinese Scorpion Buthus martensii Karsch
Author(s) -
Zeng XianChun,
Li WenXin,
Peng Fang,
Zhu ZhiHui
Publication year - 2000
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/713803610
Subject(s) - peptide , signal peptide , complementary dna , peptide sequence , venom , biochemistry , amino acid , microbiology and biotechnology , biology , chemistry , gene
Based on the amino acid sequence of a bradykinin‐potentiating peptide (Bpp) (peptide K‐12) from scorpion Buthus occitanus, a full‐length cDNA sequence encoding the precursor of a novel venom peptide (named BmKbpp) related to this Bpp, has been isolated and analyzed. The cDNA encodes a precursor of 72 amino acid residues, including a signal peptide of 22 residues and an extra Arg‐ArgArg tail at the C‐terminal end of the precursor, which have to be removed in the processing step. The C‐terminal region (21 residues) of the precursor is homologous (57% identical) with the sequence of peptide K‐12. Thus, according to the primary structure of the BmKbpp precursor, there may be a propeptide between the signal peptide and the putative mature BmKbpp at the C‐terminal region of the precursor.