Premium
Characterization of Cytostatically Active Glycosphingolipids Isolated from Thioglycollate‐Elicited Murine Macrophages
Author(s) -
Schaade Lars,
Ritter Klaus,
Schiebel HansMartin,
Thomssen Reiner,
Kleines Michael
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/713803512
Subject(s) - sphingosine , ceramide , chemistry , glycosphingolipid , moiety , oligosaccharide , membrane , biochemistry , ganglioside , stereochemistry , receptor , apoptosis
Two acidic glycosphingolipids (gangliosides) derived from mouse macrophage membranes and separated by thin‐layer chromatography have a strong cytostatic effect on human and mouse tumor cells. The structure of the two gangliosides, named MphiG1 and MphiG2, was elucidated by application of physicochemical and immunochemical methods. Gas chromatography and mass spectrometry of MphiG1 and MphiG2 classified them as isomeric monosialogangliosides with ceramide moieties composed of sphingosine as the long‐chain base, C16 and C18 fatty acids, respectively, and a lacto‐tetraose backbone. For MphiG1, additional immunochemical findings led to the proposed structure IV3NeuAc‐nLcOse4Cer. The immunochemical reactions of MphiG2 suggest a branched structure for the oligosaccharide moiety.