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New Chemiluminescence Assay for Linamarin
Author(s) -
Ruengprapavut Sutarnthip,
Chulavatnatol Montri
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/713803500
Subject(s) - chemiluminescence , luminol , chemistry , glucose oxidase , chromatography , peroxidase , biochemistry , vanadate , enzyme
A new chemiluminescence assay was developed for the quantitative determination of linamarin, a cyanogenic glucoside present in cassava. The assay involved hydrolysis of linamarin by a specific enzyme, linamarase, to release glucose, which was then quantitated by a chemiluminescence system consisting of glucose oxidaseperoxidase‐luminol.The new assay was more sensitive than the conventional spectrophotometric method for quantitating linamarin in cassava extracts. However, the following agents were found to interfere with the new assay:Vanadate,Mg2+,and Cu2+,were inhibitory to the luminescence of the H2O2‐peroxidase‐luminol system used in the coupling reaction, whereas EDTA and EGTA activated the system. In addition, Hg2+, which inhibits glucose oxidase, and Tris ion, which inhibits linamarase, both interfered with the new assay.