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Suppression of Caspase‐3 and Nitric‐Oxide Synthase‐2 During Buccal Mucosal Ulcer Healing: Effect of Chronic Alcohol Ingestion
Author(s) -
Slomiany B. L.,
Piotrowski J.,
Slomiany A.
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/713803479
Subject(s) - nitric oxide synthase , apoptosis , caspase 3 , alcohol , nitric oxide , ethanol , ingestion , medicine , protease , endocrinology , chemistry , immunology , biochemistry , enzyme , programmed cell death
Abstract In this study, we analyzed the effect of chronic alcohol ingestion on the expression of constitutive (cNOS) and inducible (NOS2) nitric‐oxide synthase and the activity of an apoptotic protease, caspase‐3, during buccal mucosal ulcer healing in rats maintained for 5 weeks on alcohol‐containing or control liquid diet. In comparison with the controls, the ulcer onset in the alcohol group was characterized by a 2.5‐fold greater epithelial cells apoptosis, 2.1‐fold greater expression of caspase‐3 activity, and a 1.4‐fold greater enhancement in NOS‐2, but expression of cNOS showed a 1.3‐fold decrease. In both groups the ulcer healing was accompanied by a gradual decline in apoptosis, caspase‐3, and NOS‐2 and a recovery in cNOS activity, but the changes were considerably slower in the alcohol diet group, as manifested by a 40%(4 days) delay in ulcer healing. These results suggest that chronic alcohol ingestion interferes with the suppression of NOS‐2 and the apoptotic events propagated by caspase‐3 and hence affects the efficiency of oral mucosal repair process.