Open AccessNeuroblastoma‐secreted exosomes carrying miR‐375 promote osteogenic differentiation of bone‐marrow mesenchymal stromal cells
Author(s) -
Colletti Marta,
Tomao Luigi,
Galardi Angela,
Paolini Alessandro,
Di Paolo Virginia,
De Stefanis Cristiano,
Mascio Paolo,
Nazio Francesca,
Petrini Stefania,
Castellano Aurora,
Russo Ida,
Caruso Roberta,
Piga Simone,
De Vito Rita,
Pascucci Luisa,
Peinado Hector,
Masotti Andrea,
Locatelli Franco,
Di Giannatale Angela
Publication year - 2020
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.1080/20013078.2020.1774144
Subject(s) - microvesicles , mesenchymal stem cell , stromal cell , bone marrow , cancer research , neuroblastoma , metastasis , exosome , tumor microenvironment , biology , microrna , medicine , immunology , pathology , cell culture , microbiology and biotechnology , cancer , tumor cells , biochemistry , genetics , gene
Bone marrow (BM) is the major target organ for neuroblastoma (NB) metastasis and its involvement is associated with poor outcome. Yet, the mechanism by which NB cells invade BM is largely unknown. Tumour microenvironment represents a key element in tumour progression and mesenchymal stromal cells (MSCs) have been recognized as a fundamental part of the associated tumour stroma. Here, we show that BM‐MSCs isolated from NB patients with BM involvement exhibit a greater osteogenic potential than MSCs from non‐infiltrated BM. We show that BM metastasis‐derived NB‐cell lines secrete higher levels of exosomal miR‐375, which promotes osteogenic differentiation in MSCs. Of note, clinical data demonstrate that high level of miR‐375 correlates with BM metastasis in NB patients. Our findings suggest, indeed, a potential role for exosomal miR‐375 in determining a favourable microenvironment in BM to promote metastatic progression. MiR‐375 may, thus, represent a novel biomarker and a potential target for NB patients with BM involvement.