Open AccessExtracellular vesicles provide a capsid‐free vector for oncolytic adenoviral DNA delivery
Author(s) -
Saari Heikki,
Turunen Tiia,
Lõhmus Andres,
Turunen Mikko,
Jalasvuori Matti,
Butcher Sarah J.,
YläHerttuala Seppo,
Viitala Tapani,
Cerullo Vincenzo,
Siljander Pia R. M.,
Yliperttula Marjo
Publication year - 2020
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.1080/20013078.2020.1747206
Subject(s) - oncolytic virus , lytic cycle , microvesicles , oncolytic adenovirus , cancer cell , biology , gene delivery , extracellular vesicles , viral vector , extracellular vesicle , virology , genetic enhancement , cancer , virus , microbiology and biotechnology , gene , microrna , genetics , recombinant dna , biochemistry
Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses for cancer treatment. Delivery of oncolytic viruses in EVs could provide considerable advantages, hiding the viruses from the immune system and providing alternative entry pathways into cancer cells. Here we describe the formation and viral cargo of EVs secreted by cancer cells infected with an oncolytic adenovirus (IEVs, infected cell‐derived EVs) as a function of time after infection. IEVs were secreted already before the lytic release of virions and their structure resembled normally secreted EVs, suggesting that they were not just apoptotic fragments of infected cells. IEVs were able to carry the viral genome and induce infection in other cancer cells. As such, the role of EVs in the life cycle of adenoviruses may be an important part of a successful infection and may also be harnessed for cancer‐ and gene therapy.