Open AccessDetermination of variability due to biological and technical variation in urinary extracellular vesicles as a crucial step in biomarker discovery studies
Author(s) -
Oeyen Eline,
Willems Hanny,
'T Kindt Ruben,
Sandra Koen,
Boonen Kurt,
Hoekx Lucien,
De Wachter Stefan,
Ameye Filip,
Mertens Inge
Publication year - 2019
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.1080/20013078.2019.1676035
Subject(s) - biomarker discovery , biomarker , extracellular vesicles , urine , microvesicles , ultrafiltration (renal) , urinary system , extracellular vesicle , computational biology , chromatography , chemistry , bioinformatics , biology , proteomics , biochemistry , microrna , microbiology and biotechnology , gene , endocrinology
Urinary extracellular vesicles (EVs) are an attractive source of biomarkers for urological diseases. A crucial step in biomarker discovery studies is the determination of the variation parameters to perform a sample size calculation. In this way, a biomarker discovery study with sufficient statistical power can be performed to obtain biologically significant biomarkers. Here, a variation study was performed on both the protein and lipid content of urinary EVs of healthy individuals, aged between 52 and 69 years. Ultrafiltration (UF) in combination with size exclusion chromatography (SEC) was used to isolate the EVs from urine. Different experimental variation set‐ups were used in this variation study. The calculated standard deviations (SDs) of the 90% least variable peptides and lipids did not exceed 2 and 1.2, respectively. These parameters can be used in a sample size calculation for a well‐designed biomarker discovery study at the cargo of EVs.