Open AccessMicrosatellite Mixed‐Stock Identification of Coho Salmon in British Columbia
Author(s) -
Beacham Terry D.,
Candy John R.,
Wallace Colin,
Wetklo Michael,
Deng Langtuo,
MacConnachie Cathy
Publication year - 2012
Publication title -
marine and coastal fisheries
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 28
ISSN - 1942-5120
DOI - 10.1080/19425120.2012.661393
Subject(s) - stock (firearms) , oncorhynchus , microsatellite , fishery , population , geography , biology , allele , demography , archaeology , fish <actinopterygii> , biochemistry , sociology , gene
Variation at 17 microsatellite loci was analyzed for about 50,000 coho salmon Oncorhynchus kisutch sampled from 274 locations ranging from Russia to California (but largely from British Columbia), and the variation was applied to estimate stock composition in mixed‐stock fishery samples. High resolution of mixed‐stock samples was possible; accurate estimates of stock composition were available for coho salmon originating from 39 regions (Russia, 1 region; Yukon River, 1; southeast Alaska, 1; British Columbia, 28; Washington, 5; Columbia River, 1; Oregon, 1; California, 1). The power of a locus in providing accurate estimates of stock composition of simulated single‐population mixtures was related to the number of alleles observed at the locus. Approximately 800 alleles were observed across the 17 microsatellites. Analysis of known‐origin samples indicated that accurate regional estimates of stock composition were obtained; estimates from 37 of 39 regions had accuracy greater than 90%. Estimated stock compositions of five mixed‐fishery samples collected in British Columbia and the San Juan Islands (Washington) reflected the presence and timing of migration of the local populations. Microsatellites provided accurate estimates of stock composition from many locations in the British Columbia distribution of coho salmon. Received July 5, 2011; accepted October 22, 2011