Estimating Oocyte Growth Rate and Its Potential Relationship to Spawning Frequency in Teleosts with Indeterminate Fecundity

Abstract We present a method for estimating the interspawning interval (ISI) in batch‐spawning teleosts with indeterminate fecundity based on the rate of oocyte growth and the size of oocytes at the beginning and end of the spawning cycle. The method is accompanied by a number of prerequisites, which are tested and subsequently applied to wild collections of Atlantic sardine Sardina pilchardus (also known as European pilchard). The rate of oocyte growth and oocyte size at the end of vitellogenesis were shown to exhibit rather constant values in Atlantic sardine; thus, the ISI could be simply estimated as a factor of oocyte size at the beginning of the spawning cycle ( O b ). Given that vitellogenesis in Atlantic sardine ceases at final oocyte maturation, O b was estimated by measuring the size of oocytes of the subsequent batch in females with hydrated oocytes. The resulting average ISI was very close to inverse values of the spawning fraction estimated through the postovulatory follicle method, which indicated the validity of the ISI method. Applications of the ISI method require fewer samples of adult females compared with the postovulatory follicle method, are histology independent, and could be combined with oocyte counts to provide batch fecundity measurements. These modifications in spawning frequency and batch fecundity estimation could help to decrease both cost and labor in daily egg production method surveys.