Open AccessAnti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells
Author(s) -
Gi-Chang Kim,
Jinsook Kim,
Gyoung-Mi Kim,
Song-Yi Choi
Publication year - 2017
Publication title -
food and nutrition research/food and nutrition research. supplement
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 37
eISSN - 1654-6628
pISSN - 1654-661X
DOI - 10.1080/16546628.2017.1339555
Subject(s) - adipogenesis , ethanol , biology , botany , chemistry , traditional medicine , biochemistry , medicine , in vitro
Background : Edible flowers, Tropaeolum majus has been used as a disinfectant and an antibiotic, and for wound healing, but the anti-obesity effects of this plant have not been reported previously Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid accumulation levels were determined using Oil-Red O staining and a triglyceride content assay. Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. Results : The highest inhibition of lipid accumulation was observed at a TME concentration of 300 µg/mL. Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. This decrease was shown to be concentration-dependent. Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. Conclusions : TME may be a potential novel therapeutic agent for the prevention and treatment of obesity.