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Ploidy Variation and Viability of Aneuploid Ornamental Koi Carp Obtained by Crossing Triploid Females with Diploid Males
Author(s) -
Gomelsky Boris,
Delomas Thomas A.,
Warner Jeffrey L.
Publication year - 2016
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1080/15222055.2016.1150923
Subject(s) - biology , ploidy , hatching , cyprinus , reproduction , zoology , carp , fish <actinopterygii> , larva , common carp , catfish , botany , ecology , genetics , fishery , gene
Results of crossing triploid females of ornamental koi, a variant of Common Carp Cyprinus carpio , with diploid koi males in two consecutive spawning seasons (2014 and 2015) are presented. A total of seven progenies from six triploid females (one female was spawned twice) were produced and analyzed. The same as normal diploid females, triploid females were highly fertile and produced hundreds of thousands of eggs (up to 400,000 per female). Processes of embryo incubation and hatching of larvae in progenies obtained from triploid females proceeded normally; however, mass mortality of hatched larvae occurred at the swim‐up stage. Nevertheless about 200,000 swim‐up larvae were obtained and stocked for further rearing. A total of about 1,000 juveniles (or 0.5% of the number of stocked larvae) was collected from outdoor tanks. As expected, all analyzed larvae and juveniles in control progenies obtained by crosses of diploid females with diploid males were diploid. About 95% of analyzed fish (larvae and juveniles) obtained by crossing triploid females with diploid males were aneuploids with ploidy ranging from 2.14n to 3.0n; mean values of fish ploidy in progenies obtained from different triploid females varied from 2.47n to 2.63n. Since aneuploid fish have in their genomes one haploid set from parental males, the data obtained indicate that triploid koi females produced aneuploid eggs with ploidy range from haploid to diploid level and a modal ploidy level around 1.5n, similar to the production of aneuploid spermatozoa observed earlier for triploid males in fish. About 5% of juveniles obtained from triploid females had ploidy range from 3.21n to 4.0n. Apparently these fish resulted from spontaneous suppression of the second meiotic division in aneuploid eggs.