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Sperm Quality of Hatchery‐Reared Lake Trout Throughout the Spawning Season
Author(s) -
Johnson Katelynn,
Butts Ian A. E.,
Wilson Chris C.,
Pitcher Trevor E.
Publication year - 2013
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1080/15222055.2012.711277
Subject(s) - hatchery , biology , trout , sperm , human fertilization , longevity , seasonal breeder , salvelinus , zoology , reproduction , seasonality , sperm motility , ecology , fishery , fish <actinopterygii> , botany , anatomy , genetics
The objective of this study was to investigate variation in sperm quality metrics (motility, velocity, linearity, longevity, and density) of hatchery‐reared Lake Trout Salvelinus namaycush throughout the spawning season. Seasonal variation in sperm quality was investigated using both a regression and repeated‐measures approach. Sperm was collected from the same 16 individuals over four sampling periods, separated by 3‐week intervals. Regression analyses showed that 7–27% of the variation in sperm traits could be explained by seasonal variation, indicating that seasonality can have a significant impact on the quality of sperm. Significant positive linear relationships were found for percent motility and linearity at 5 s postactivation. Significant negative quadratic relationships were found for velocity at 5 s postactivation, longevity, and density, whereas a positive quadratic relationship was found for linearity at 10 s postactivation. Repeated measures ANOVAs showed a significant effect of season for percent motility and linearity at 5 and 10 s postactivation, velocity at 10 s postactivation, and longevity. Our findings are important for optimizing fertilization protocols for hatchery production and can also be used to understand reproductive biology and ecology of wild Lake Trout stocks.