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Efficacy of a Commercial Probiotic Relative to Oxytetracycline as Gram‐Negative Bacterial Control Agents in a Rotifer ( Brachionus plicatilis ) Batch Culture
Author(s) -
Rotman Federico J.,
Riche Marty,
Van Wyk Peter,
Benetti Daniel D.
Publication year - 2011
Publication title -
north american journal of aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 41
eISSN - 1548-8454
pISSN - 1522-2055
DOI - 10.1080/15222055.2011.603968
Subject(s) - rotifer , biology , brachionus , probiotic , oxytetracycline , microbiology and biotechnology , lactobacillus , food science , antimicrobial , zoology , antibiotics , veterinary medicine , ecology , bacteria , fermentation , medicine , genetics
Two trials were conducted to evaluate two gram‐negative bacterial control strategies in batch cultures of the rotifer Brachionus plicatilis . In the first trial, rotifers at an initial density of 47/mL were cultured for 5 d and dosed with a 10‐mg/L solution of either oxytetracycline or a commercial probiotic consisting principally of Bacillus and Lactobacillus . In the second trial, rotifers at an initial density of 140/mL were cultured for 4 d and dosed with 20‐mg/L solutions of oxytetracycline or the probiotic. Rotifer density, egg counts, and egg : female ratios were determined daily. At the termination of trial 1 and on days 0, 2, and 4 of trial 2, counts of bacterial colonies were conducted to estimate bacterial loads in the rotifer culture water. Oxytetracycline was an effective antimicrobial and enhanced rotifer production in the higher‐density culture but had only a marginal effect on production in the lower‐density culture. Conversely, inoculation of the low‐density culture with the probiotic had no effect on rotifer production. Inoculation of the high‐density culture adversely affected rotifer production by increasing mortality and reproductive inhibition.