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Overexpression of human testis antigens in Escherichia coli host cells is influenced by site of expression and the induction temperature
Author(s) -
Mburu D. N.,
Roberts T. K.,
Boettcher B.
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900202133
Subject(s) - periplasmic space , escherichia coli , complementary dna , biology , cytoplasm , antigen , fusion protein , microbiology and biotechnology , gene expression , host (biology) , gene , biochemistry , recombinant dna , genetics
10.1080/15216549900202133.abs A panel of twenty human testis cDNA clones were expressed in an Escherichia coli expression system and six clones were found to express identifiable fusion polypeptides. Expression was found to be influenced not only by the site of localization of the polypeptide in the host cells, but also by the temperature used for induction. This emphasized the need for cytoplasmic and periplasmic expression of new antigens of unknown properties, as well as the use of temperatures of 30°C or lower. A majority of the expressed polypeptides were mainly in an insoluble form. By reducing the induction temperature to 30°C production of the soluble fraction was further improved.