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Influence of atropine on carbachol dual effect on Ca2 mobilization in SH‐SY5Y neuroblastoma cells
Author(s) -
Oras Aldo,
Järv Jaak,
Åkerman Karl E. O.
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201823
Subject(s) - carbachol , atropine , agonist , muscarinic acetylcholine receptor , antagonist , endocrinology , muscarinic antagonist , chemistry , sh sy5y , medicine , neuroblastoma , pharmacology , receptor , biology , cell culture , genetics
The muscarinic receptor stimulated mobilisation of calcium ions in SH‐SY5Y neuroblastoma cells was measured as a function on carbachol and atropine concentrations. The combined application of this pair of muscarinic agonist and antagonist yielded a set of bell‐shaped dose‐response curves. In the presence of atropine the cell responses were smaller and the up‐going phase of these relationships was shifted towards higher agonist concentration, while the down‐going phase of these curves was not influenced by the antagonist. These results pointed to a similar mechanism of the receptor inhibition at high carbachol (agonist) concentrations and by atropine (antagonist).