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Lipid peroxidation induced by the Cu,Zn‐superoxide dismutase and hydrogen peroxide system
Author(s) -
Kwon Oh Bin,
Kang Jung Hoon
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201703
Subject(s) - lipid peroxidation , chemistry , superoxide dismutase , hydrogen peroxide , hydroxyl radical , radical , liposome , biochemistry , antioxidant , dismutase , nuclear chemistry
Cu,Zn‐superoxide dismutase (SOD) can catalyze hydroxyl radical generation using H2O2 as a substrate. Lipid peroxidation induced by the Cu,Zn‐SOD and H2O2 system was investigated. When linoleic acids micelles or phosphatidylcholine liposomes were incubated with Cu,Zn‐SOD and H2O2, lipid peroxidation was gradually increased in a time‐dependent manner. The extent of lipid peroxidation was proportional to Cu,Zn‐SOD and H2O2 concentrations. Hydroxyl radical scavengers and copper chelator inhibited lipid peroxidation induced by the Cu,Zn‐SOD and H2O2 system. These results suggest that lipid peroxidation is mediated by the Cu,Zn‐SOD and H2O2 system via the generation of hydroxyl radicals by a combination of the peroxidative reaction of Cu,Zn‐SOD and the Fenton‐like reaction of free copper released from oxidatively damaged SOD.