Premium
Orthophosphate is a non‐essential activator of Vigna radiata flavokinase
Author(s) -
DasPanja Kaberi,
Jonnalagadda Vidya S.,
Jonnalagadda Sobhanaditya
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201583
Subject(s) - vigna , activator (genetics) , pi , radiata , phosphate , chemistry , phosphorylation , biochemistry , stereochemistry , biophysics , biology , botany , receptor
The ATP‐dependent phosphorylation of riboflavin to FMN by flavokinase from Vigna radiata was activated by orthophosphate (Pi) in a concentration dependent manner. Pi affected both the Km and Vmax, indicating that it is a non‐essential, mixed type activator. The extent of activation by Pi was dependent on the cation (Mg2+ or Zn2+). Activation by other anions could be correlated to similarity to Pi in molecular size and structure. These observations suggest the presence of a binding site(s) for a phosphate‐like anion on flavokinase.