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Modulation by nitric oxide (NO) of capsaicin‐induced calcium uptake into rat dorsal root ganglion neurons
Author(s) -
Park YoungHo,
Surh YoungJoon,
Lee SangSup
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201463
Subject(s) - capsaicin , dorsal root ganglion , chemistry , sodium nitroprusside , nitric oxide , calcium , stimulation , spermine , pharmacology , biophysics , depolarization , dithiothreitol , biochemistry , medicine , dorsum , anatomy , biology , receptor , enzyme , organic chemistry
The regulatory role of nitric oxide in capsaicin‐induced 45Ca2+ accumulation in dorsal root ganglion neuronal cultures was investigated. Capsaicin‐activated calcium entry was subject to complicated tuning by NO‐releasing agents sodium nitroprusside, spermine/NO complex and NO synthase inhibitor NG‐nitro‐L‐arginine methyl ester in concentration and stimulation protocol‐dependent manner. In contrast, these agents failed to change depolarization‐induced calcium influx. In experiments using dithiothreitol and 5,5‐dithio‐bis‐2‐nitrobenzoic acid this modulation was independent of the oxidizing action of NO. It is suggested that NO exerts a novel feedback modulatory effects on capsaicin‐induced calcium entry into rat DRG neurons.