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Isolation and characterization of cDNA clone for human liver 10‐formyltetrahydrofolate dehydrogenase
Author(s) -
Hong Mihui,
Lee Younghee,
Kim Jae Wha,
Lim JongSeok,
Chang Sung Yeoul,
Lee Kwang Soo,
Paik SangGi,
Choe In Seong
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201433
Subject(s) - complementary dna , cdna library , microbiology and biotechnology , open reading frame , homology (biology) , northern blot , biology , amino acid , clone (java method) , messenger rna , human liver , southern blot , peptide sequence , molecular cloning , gene , biochemistry , enzyme
A cDNA clone encoding 10‐formyltetrahydrofolate dehydrogenase (10‐FTHFDH) was isolated from a human fetal liver cDNA library. It contained the open reading frame of 2,709 base pairs and predicted a protein comprising 902 amino acids with a calculated molecular weight of 98,700 Da. The deduced protein showed about 93.6% homology (90.5% identity, 3.1% favored substitutions) when compared with rat 10‐FTHFDH. The distribution of 10‐FTHFDH transcript in various human tissues was studied by Northern blot analysis using poly(A+) RNAs from different tissues. The 10‐FTHFDH transcript with an approximate size of 2.7 kb was mainly expressed in human kidney, skeletal muscle, and liver and rarely expressed in other tissues.