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Structural characteristics of tenecin 3, an insect antifungal protein
Author(s) -
Lee YoungTae,
Kim DaeHee,
Suh JeongYong,
Chung Jae Hoon,
Lee Bok Leul,
Lee Younghoon,
Choi ByongSeok
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201393
Subject(s) - circular dichroism , antifungal , sodium dodecyl sulfate , micelle , chemistry , candida albicans , insect , biochemistry , stereochemistry , biophysics , microbiology and biotechnology , biology , organic chemistry , botany , aqueous solution
Tenecin 3, an antifungal protein, previously isolated from the insect Tenebrio molitor, inhibits growth of the fungus Candida albicans. However, the antifungal mechanism and functions of tenecin 3 remain unknown. As an initial step to study the mechanism and functions, physical and structural properties of tenecin 3 were examined by circular dichroism (CD) analysis and 2D nuclear overhauser effect spectroscopy. These analyses suggest that tenecin 3 has a propensity of random structure with very loose turn‐like elements. The CD results also indicate that this random structural propensity is not significantly affected by temperature, pH, and by the presence of organic solvents or sodium dodecyl sulfate (SDS) micelles. However, the hydrodynamic studies suggest that tenecin 3 is not in extended form in spite of its random structural feature.