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Cloning and expression of bovine herpesvirus‐1 glycoprotein C
Author(s) -
Gupta Praveen K.,
Saini Mohini,
Gupta L. K.,
Bandyopadhyay S. K.,
Garg S. K.
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201293
Subject(s) - bovine herpesvirus 1 , ecori , microbiology and biotechnology , biology , recombinant dna , virology , plasmid , transfection , immunofluorescence , cloning (programming) , virus , glycoprotein , gene , antibody , herpesviridae , genetics , viral disease , computer science , programming language
Abstract Glycoprotein C (gC) of Bovine Herpesvirus‐1 (BHV‐1) is expressed at high levels on surface of infected cells and on virus envelope. It is relatively immunodominant in antibody response to BHV‐1 infection and protective in immunized bovines against BHV‐1 challenge. In an attempt to express gC in mammalian cells, the 2.4 kb BamHI‐EcoRI fragment, containing complete coding sequence of the gC gene was excised from a recombinant plasmid and cloned under the control of RSV‐LTR. The resultant plasmid pRSV‐gC was transfected into MDBK cells and expression of gC was detected by indirect immunofluorescence. The non‐permeabilized cells revealed surface expression of gC.