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CYP2B2 gene expression and phenobarbital induction in kidneys using an in vitro transcription system
Author(s) -
Shervington Amal
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201243
Subject(s) - transcription (linguistics) , phenobarbital , biology , gene , gene expression , microbiology and biotechnology , in vitro , transcriptional regulation , transcription factor , promoter , genetics , pharmacology , philosophy , linguistics
The induction of cytochrome P450 2B1 and 2B2 in rat liver in response to phenobarbital is mediated at the transcriptional level. While in extrahepatic tissue such as the kidney, the transcription level of the CYP 2B2 gene is much lower than in the liver. The transcriptional activity of the CYP2B2 promoter constructs in nuclear proteins from kidney revealed that the in vitro system maintained the tissue‐specificity of CYP2B2 transcriptional induction but not that of the basal transcription of the gene.