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Effects of sulfhydryl compounds on the inhibition of erythrocyte membrane Na ‐K ATPase by ozone
Author(s) -
Bilgin Ramazan,
Gül Sermin,
Tükel S. Seyhan
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201233
Subject(s) - membrane , chemistry , enzyme , ozone , atpase , thiol , in vitro , biochemistry , enzyme assay , organic chemistry
Exposure of human erythrocyte membranes to ozone (5 μmol/10 min) resulted in the inhibition of erythrocyte membrane Na+‐K+ ATPase (EC.3.6.1.39). It was determined that, the degree of enzyme inhibition in the directly ozone exposed membranes was greater than that of membranes obtained from ozone exposed intact erythrocytes. In the presence of varying concentrations (0‐1.0 mM) of dithiotrethiol or mercaptoethanol Na+‐K+ ATPase activities of both types of ozone exposed membranes were increased almost proportionally with the concentration of dithiotrethiol or mercaptoethanol however, the activities were still lower than the normal Na+‐K+ ATPase value. The results indicate that, dithiotrethiol or mercaptoethanol prevent the enzyme inhibition by ozone in vitro. This suggests that the membrane thiol groups are primary targets for ozone and thereby preventing the oxidation of essential functional groups of enzyme, protein.