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Cloning of a tellurite resistance determinant from Bacillus stearothermophilus V in Escherichia coli
Author(s) -
Vásquez Claudio,
Saavedra Claudia,
Loyola Claudia,
Moscoso Hugo,
Pichuantes Sergio
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201173
Subject(s) - escherichia coli , cloning (programming) , bacillus (shape) , microbiology and biotechnology , biology , molecular cloning , dna , chemistry , genetics , gene , peptide sequence , computer science , programming language
A potassium tellurite‐resistance determinant was isolated from Bacillus stearothermophilus V and cloned in Escherichia coli. Transformed cells formed black colonies when grown on solid media containing permissive tellurite concentrations. The resistance determinant was contained in a B. stearothermophilus V chromosomal DNA fragment of 7 kb.