Premium
Differential regulation of protooncogene c‐myc expression in rat ventral prostate after castration
Author(s) -
Lee JoongHwa,
Sul ChongKoo,
Kim YoungKyun,
Hwang ByungDoo,
Lim Kyu
Publication year - 1999
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549900201143
Subject(s) - castration , messenger rna , cycloheximide , androgen , northern blot , endocrinology , medicine , prostate , biology , in situ hybridization , gene expression , andrology , gene , microbiology and biotechnology , protein biosynthesis , hormone , genetics , cancer
To evaluate the possibility that the protooncogene c‐myc plays a role in ventral prostate, the effects of castration have been investigated at a beginning of a period by Northern blot hybridization and the levels of c‐myc mRNA were also compared with mRNA of androgen‐regulated genes, C1 and TRPM‐2. Levels of c‐myc mRNA in ventral prostate increased with maximal stimulation reached at 6 hours (early induction) and 48 hours (late induction) after castration, respectively. The level of C 1 mRNA did not change and TRPM‐2 was not detected at early induction of c‐myc mRNA after castration. The level of early induction of c‐myc mRNA after castration was increased in ventral prostate treated with cycloheximide, but it was almost reduced by actinomycin‐D pretreatment. Administration of androgen at the time of castration prevented early induction of c‐myc mRNA. These results suggest that protooncogene c‐myc is differentially regulated in ventral prostate after castration.