Premium
Gene transfer via pollen‐tube pathway for anti‐fusarium wilt in watermelon
Author(s) -
Chen WenShaw,
Chiu ChienChih,
Liu HoYih,
Lee TanLon,
Cheng JiinTsuey,
Lin ChengChung,
Wu YueJean,
Chang HueiYu
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204762
Subject(s) - pollen tube , fusarium wilt , gene transfer , gene , biology , pollen , tube (container) , botany , genetics , fusarium oxysporum , materials science , pollination , composite material
In order to obtain transgenic fusarium wilt resistant watermelon plants, squash DNA was introduced into the ovaries of watermelon plants via the pollen‐tube pathway. The introduction of foreign genes into ovaries was accomplished using co‐transformation with the CaMV35S‐GUS as a marker. Transformed watermelon plants contained integrated copies of the GUS activity and the seeds of transformed progeny produced a blue color when stained with 5‐bromo‐4‐chloro‐3‐indolyl glucuronide, whereas seeds from untransformed control plants did not. Of 200 transformed seedlings, ten were wilt resistant. The presence of the GUS activity in the genome of stable transgenic seedlings was confirmed by Southern blot analysis. Furthermore, the generation of random amplified polymorphic DNA (RAPD) fingerprints using primers with embedded restriction sites showed amplification products unique to these transgenic plants. Primers OPA‐1 and OPA‐9 gave distinct band patterns of genomic DNA using the polymerase chain reaction.