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Sphingosine induces phospholipase D and mitogen activated protein kinase in vascular smooth muscle cells
Author(s) -
Taher Mohiuddin M.,
AbdElfattah Anwar S.,
Sholley Milton M.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204552
Subject(s) - phosphatidic acid , pld2 , diacylglycerol kinase , sphingosine , phospholipase d , microbiology and biotechnology , vascular smooth muscle , protein kinase c , protein kinase a , phosphatidylethanol , lipid signaling , biochemistry , biology , phospholipase , chemistry , kinase , signal transduction , endocrinology , phospholipid , enzyme , smooth muscle , receptor , membrane
The enzymes phospholipase D and diacylglycerol kinase generate phosphatidic acid which is considered to be a mitogen. Here we report that sphingosine produced a significant amount of phosphatidic acid in vascular smooth muscle cells from the rat aorta. The diacylglycerol kinase inhibitor R59 949 partially depressed sphingosine induced phosphatidic acid formation, suggesting that activation of phospholipase C and diacylglycerol kinase can not account for the bulk of phosphatidic acid produced and that additional pathways such as phospholipase D may contribute to this. Further, we have shown that phosphatidylethanol was produced by sphingosine when vascular smooth muscle cells were stimulated in the presence of ethanol. Finally, as previously shown for other cell types, sphingosine stimulated mitogen‐activated protein kinase in vascular smooth muscle cells.