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Protein Binding in vivo to OP2 promoter of the Pseudomonas putida TOL plasmid
Author(s) -
Miura Koshiro,
Inouye Sachiye,
Nakazawa Atsushi
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204482
Subject(s) - chemistry , transcription (linguistics) , pseudomonas putida , biochemistry , dna footprinting , rna polymerase , catabolism , microbiology and biotechnology , plasmid , dna , rna , enzyme , biology , gene , promoter , gene expression , philosophy , linguistics
The transcription of OP2 encoding enzymes for m‐toluate catabolism on the Pseudomonasputida TOL plasmid is activated by basal‐level XylS protein in the presence of m‐toluate or by overproduced XylS protein in the absence of m‐toluate. In this study, in vivo dimethyl sulfate (DMS) footprinting was performed to understand the mechanism of transcriptional regulation of OP2 promoter by XylS. In the presence of overproduced XylS without m‐toluate, several protected nucleotides were observed, indicating the binding of RNA polymerase to DNA. However, the protection was canceled upon addition of m‐toluate. These results suggest that RNA polymerase is retained by XylS on the OP2 promoter in the absence of inducer, and is released by m‐toluate binding to XylS, concomitant with transcription.