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Endothelin 1 action on isolated rat stomach and the role of calcium ions in ET 1 induced depolarization of smooth muscle cells BC3H1
Author(s) -
Hukovic N.,
Hadziselimovic R.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204422
Subject(s) - depolarization , extracellular , endothelin receptor , endothelins , medicine , endocrinology , stimulation , intracellular , contraction (grammar) , calcium , biology , acetylcholine , vascular smooth muscle , chemistry , biophysics , microbiology and biotechnology , smooth muscle , receptor
Endothelins; (ET1, ET2 and ET3) are a family of peptides which acts on different smooth muscle preparations inducing a slow long lasting contraction. We investigated the effects of ET 1 modulatory action on adrenergic, cholinergic and serotoninergic transmission on an isolated mouse's stomach with gastric nerves. The endothetin 1 stimulation of the mouse stomach tone was abolished by the specific serotonin antagonist methizergid. This study suggests that endothelin 1 plays a role in the regulation of nonvascular smooth muscle tone. The endothelin effect was dependent on free intracellular Ca++ which can be recruited from an extracellular solution as well as from intracellular stores. Complete reduction of Ca++ from the extracellular solution with a simultaneous depletion of calcium stores abolished endothelin 1 depolarization of BC3H1 cells.