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Occurrence of bovine spleen CD38/NAD glycohydrolase disulfide‐linked dimers
Author(s) -
Berruet Laure,
MullerSteffner Hélène,
Schuber Francis
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204392
Subject(s) - nad+ kinase , enzyme , chemistry , biochemistry , cd38 , polyclonal antibodies , monomer , stereochemistry , cyclic adp ribose , antibody , biology , organic chemistry , stem cell , cd34 , immunology , genetics , polymer
Bovine spleen NAD+ glycohydrolase, an ecto‐enzyme closely related to CD38, catalyzes the conversion of NAD+ into ADP‐ribose and cyclic ADP‐ribose, a calcium‐mobilizing metabolite. We have raised polyclonal antibodies against the native enzyme which on immunoblots revealed, besides the 32 kDa monomer, the presence of a stable dimeric form. This dimerization was shown to result from a spontaneous oxidative process involving the formation of one or several disulfide bond(s) sensitive to reducing agents such as 2‐mercaptoethanol. The homodimeric oxidized enzyme, which was not detected during the early steps of the enzyme purification procedure, was catalytically active. Our results underline the differences, in terms of oligomerization and reactivity towards thiols, between CD38/NAD+ glycohydrolases depending on their origin.