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cDNA cloning and sequence analysis of six neurotoxin‐like proteins from Chinese continental banded krait
Author(s) -
Qian Youcun,
Fan Chunyang,
Gong Yi,
Yang Shengli
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204362
Subject(s) - biology , complementary dna , homology (biology) , nucleic acid sequence , phylogenetic tree , peptide sequence , sequence analysis , molecular cloning , amino acid , neurotoxin , cloning (programming) , biochemistry , microbiology and biotechnology , genetics , gene , computer science , programming language
The cDNAs encoding six neurotoxin‐like proteins were cloned from the total RNA isolated from one venom gland of Bungarus multicinctus multicinctus by reverse transcription‐polymerase chain reaction. Although they shared a high degree of nucleotide sequence homology, the deduced proteins displayed a rather low extent of identical residues with the exception that the 21 residue signal peptides were highly conserved. Each mature protein consisted of 65, 66 or 68 amino acids with four or five disulfide bonds respectively. Comparative sequence analyses showed that they were structurally related to neurotoxin homologues. Phylogenetic analyses implied that they might represent two different evolutionary branches.