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Transcription of the rat p53 gene is induced by a 39kDa protein binding to the p53 promoter region during the liver regeneration
Author(s) -
Lee Minhyung,
Park Sunhee,
Song Haisun,
Park Jongsang
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204312
Subject(s) - ap 1 transcription factor , microbiology and biotechnology , liver regeneration , transcription (linguistics) , biology , dna footprinting , gene , footprinting , promoter , transcription factor , gene expression , response element , biochemistry , regeneration (biology) , linguistics , philosophy
The adult rat liver is normally in a state of growth arrest. However, cell loss such as partial hepatectomy can induce the proliferation of the hepatocytes. Early after partial hepatectomy, the concentration of p53 mRNA increases during the prereplicative phase. In this study, we identified the cis‐regulatory element involved in the induced transcription of the rat p53 gene by DNase I footprinting assay. This element had a partial homolgy to the AP 1 recognition motif, but the competition study with AP1 oligonucleotide showed that this element was not the AP1 recognition motif. The molecular weight of the binding protein to this motif was determined as 39kDa by southwestern blotting analysis. In vitro transcription assay with the competitor containing the binding motif showed that the 39kDa protein binding to the element was requried for the induced transcription of the rat p53 gene during the liver regeneration.