Premium
Isolation of the genomic clone of the rhesus monkey beta‐amyloid precursor protein
Author(s) -
Songa Weihong,
Lahiri Debomoy K.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204302
Subject(s) - genomic dna , microbiology and biotechnology , gene , biology , southern blot , exon , genomic library , intron , promoter , clone (java method) , homology (biology) , genetics , peptide sequence , gene expression
A change in the regulation of the beta‐amyloid precursor protein (βAPP) gene may be a factor for Alzheimer's disease (AD). We have screened a rhesus monkey genomic library and pulled out a ∼17 kb genomic DNA region which contains the 5′‐flanking region (promoter), first exon and intron of the βAPP gene of the Rhesus monkey (rhβAPP). We have partially characterized the genomic clone by selective restriction enzyme digestion followed by Southern blotting against the βAPP gene‐specific DNA probes. The identity and authenticity of the different bands of the clone were also confirmed by Southern blot analysis of the rhesus monkey genomic DNA. Functional identification of the genomic fragment was determined by a promoter assay using the chloramphenicol acetyltransferase (CAT) enzyme as a reported gene. Our results showed that a 1.2 kb fragment from the 5′‐flanking region of the rhβAPP gene possessed strong promoter activity. The isolation of this genomic clone will enable characterization of the structure and function of the promoter of the rhβAPP gene. Our initial results indicate a high degree of homology between the structure of the rhesus monkey and human βAPP promoter.