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Augmentation in the expression of a ribosomal protein associated with transformation
Author(s) -
Go Hiroki,
Taniguchi ShuN′ichiro
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204152
Subject(s) - complementary dna , microbiology and biotechnology , northern blot , biology , ribosomal protein , transfection , gene , gene expression , southern blot , transcription (linguistics) , rna , genetics , ribosome , linguistics , philosophy
Using differential hybridization technique, cDNA that was a partial sequence of rat ribosomal protein L41 was isolated from fos‐ and src‐transfected rat fibroblast 3Y1. The cDNA was named #31. Subsequently, the human counterpart(named hm3168) was cloned from a human melanoma cell line(M14) and the amino acid sequence deduced from the cDNA proved to be identical to that of HG‐12. The gene expression of hm3168 was inducible by serum‐stimulation. It reveals the possible up‐regulation of the transcription via fos‐mediated signal transduction. Northern‐blot and Western‐blot analyses showed a large expression of the #31 gene in the rat transformed cell lines.