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The use of a chimera HIV‐1/HIV‐2 envelope protein for immunodiagosis of HIV infection: Its expression and purification in E. coli by use of a translation initiation site within HIV‐1 env gene
Author(s) -
Han Baoguang,
Song Xiaoguo,
Chen Qun,
Wang Hongyan,
Ling Shigan
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204132
Subject(s) - human immunodeficiency virus (hiv) , chimera (genetics) , virology , translation (biology) , biology , protein expression , eukaryotic translation , envelope (radar) , medicine , genetics , messenger rna , computer science , telecommunications , radar , gene
A chimera HIV‐1/HIV‐2 envelope sequence composed of multiple conserved immunodominant epitopes of HIV‐1 envlope protein (HIV‐1 IIIB: env482‐518+env548‐675) and the HIV‐2 gp36 immunodominant epitope (env592‐603), was constructed and directly over‐expressed in E. coli by using a prokaryotic translation initiation sequence contained within the gene of HIV‐1 envelope. The recombinant product was purified and applied in antibody‐screening assay. The purified chimera antigen reacted with all the thirty‐eight HIV‐1 positive serum samples, the two HIV‐2 serum samples, and had no cross‐reaction with all the eighty‐eight normal healthy serum sample. The results indicated that this recombinant chimera HIV‐1/HIV‐2 envelope protein could be useful for diagnostic purposes of HIV infection.