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Role of DNA definite structural elements in interaction with repair enzyme uracil‐DNA glycosylase
Author(s) -
Kubareva Elena A.,
Vasilenko Natalja L.,
Vorobjeva Olga V.,
Volkov Evgeny M.,
Oretskaya Tatjana S.,
Korshunova Galina A.,
Nevinsky Georgy A.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204122
Subject(s) - uracil dna glycosylase , dna glycosylase , uracil , dna , mutyh , chemistry , dna repair , biochemistry , enzyme
Interaction of different oligodeoxyribonucleotides (oligos), their analogs and oligonucleopeptide with uracil‐DNA glycosylase (UDG) from human placenta was investigated. It is shown that there is no considerable contribution of heterocyclic bases of DNA to UDG‐substrate binding but the UDG interaction with some DNA phosphate groups is necessary for enzyme‐substrate recognition. However the phosphate group adjacent to single dU from the 3′‐end in oligo is not involved into the electrostatic contact with UDG. It is found that UDG has the high affinity to its reaction product. An oligonucleotide containing a single 2′‐deoxy‐2′‐aminouridine is a non‐hydrolyzable substrate analog for UDG.