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Simple determination of peroxyl radical‐trapping capacity
Author(s) -
Bartosz Grzegorz,
Janaszewska Anna,
Ertel Danuta,
Bartosz Maŀgorzata
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800204042
Subject(s) - chemistry , abts , absorbance , decomposition , sulfonic acid , chromatography , antioxidant , organic chemistry , dpph
A simple spectrophotometric method of determination of peroxyl radical‐trapping capacity (PRTC) of body fluids and food products is proposed. In this method, decomposition of 2,2′‐azobis(2‐amidopropane) hydrochloride (ABAP) is the source of peroxyl and alkoxyl radicals which oxidize 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) to a green cation radical. Antioxidant present in a sample inhibit the reaction; the induction time of the reaction is proposed as a parameter enabling determination of antioxidant content. Standard assay conditions are: 20 mM ABAP and 150 μM ABTS in 0.1 M phosphate buffer, pH 7.0, at 37°C; absorbance is monitored at 414 nm. A 10‐min assay allows for determination of the induction time of appropriately diluted sample. As examples of application of this method, PRTC values of several types of beverages are reported.