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Isolation and characterization of the mouse ribosomal protein S7 gene
Author(s) -
Annilo Tarmo,
Jelina Jelena,
Pata Illar,
Metspalu Andres
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1080/15216549800203802
Subject(s) - intron , biology , gene , exon , pseudogene , genetics , ribosomal protein , start codon , polypyrimidine tract , primer extension , microbiology and biotechnology , rna splicing , ribosomal rna , alternative splicing , nucleotide , ribosome , rna , genome
We have isolated and characterized the single intron‐containing gene encoding the mouse ribosomal protein S7. The mouse 129SV S7 gene has seven exons and six introns spanning about 5000 nucleotides. The exon‐intron structure of the gene is similar to other vertebrate homologues. Southern blot analysis showed that in addition to the isolated single‐copy intron‐containing gene, there are 10‐12 members in the mouse S7 gene family, which are all most probably processed pseudogenes. The promoter region of rpS7 contains several evolutionarily conserved putative regulatory elements. The main transcription start site was mapped to a T residue within a polypyrimidine tract, 79 nucleotides upstream from AUG codon.